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E 75: 046302. 046302 Baroud, C. , Robert de Saint Vincent, M. -P. (2007b). An optical toolbox for total control of droplet microfluidics, Lab Chip 7: 1029–1033. 1039/B702472J Barton, K. D. & Subramanian, R. S. (1989). The migration of liquid drops in a vertical temperature gradient, J. Colloid Interface Sci. 133: 211–222. 1016/0021-9797(89)90294-4 Berry, D. , Heckenberg, N. R. & Rubinsztein-Dunlop, H. (2000). Effects associated with bubble formation in optical trapping, J. Mod. Opt. 47: 1575–1585.

Why molecules move along a temperature gradient, Proc. Natl. Acad. Sci. USA 103: 19678–19682. abstract Farahi, R. , Ferrell, T. L. & Thundat, T. (2005). Marangoni forces created by surface plasmon decay, Opt. Lett. 30: 616–618. , Grigoriev, R. O. & Schatz, M. F. (2003). Optical manipulation of microscale fluid flow, Phys. Rev. Lett. 91: 054501. 054501 Giglio, M. & Vendramini, A. (1974). Thermal lens effect in a binary liquid mixture: A new effect, Appl. Phys. Lett. 25: 555–557. APL/25/555/1 Grigoriev, R.

Hydrodynamic focusing is a well known phenomenon in the area of fluid mechanics thanks to Osborne Reynolds, who first used it for flow visualization in his break-through experiment and it is widely utilized as a pipe mixer in chemical technology. However, the first ‘non-academic’ microfluidic application of hydrodynamic focusing was in the area of flow cytometry, a technique for counting, examining and sorting microscopic particles suspended in a stream of fluid. Hydrodynamic focusing, where the core flow of investigated sample is sheathed by an inert fluid, is used in flow cytometry as a way to deliver the sample of suspended cells to the analyzed region in an appropriate form.

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