By Ryan T Kelly
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Hydrodynamic focusing is a well known phenomenon in the area of fluid mechanics thanks to Osborne Reynolds, who first used it for flow visualization in his break-through experiment and it is widely utilized as a pipe mixer in chemical technology. However, the first ‘non-academic’ microfluidic application of hydrodynamic focusing was in the area of flow cytometry, a technique for counting, examining and sorting microscopic particles suspended in a stream of fluid. Hydrodynamic focusing, where the core flow of investigated sample is sheathed by an inert fluid, is used in flow cytometry as a way to deliver the sample of suspended cells to the analyzed region in an appropriate form.