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In various enzymatic syntheses, water can shift the equilibrium in the direction of hydrolysis; therefore, in methods using lipase, the water produced as a by-product was Biotransformation of Underutilized Natural Resource to Valuable Compounds in Ionic Liquid: Enzymatic Synthesis of Caffeic Acid Phenethyl Ester Analogues from Immature Coffee Beans 29 Fig. 1. Conversion of caffeoylquinic acids to methyl caffeate by chlorogenate hydrolase. (A) Alcoholysis of various caffeoyl quinic aicds (compound 1) was catalysed by chlorogenate hydrolase with methanol and [bmim][NTf2] as the reaction solvent, and methyl caffeate (compound 2) was produced.

Ser. , 47, 41-52 Part 2 Electronic Applications 3 Undeniable Contribution of Aprotic Room Temperature Ionic Liquids in the Security of Li-Ion Batteries Claude-Montigny Bénédicte, Stefan Claudia Simona and Violleau David Université François Rabelais, Laboratoire PCMB France 1. Introduction Nowadays, lithium-ion batteries are key components for portable electric devices for applications between 0°C and 50°C. Between the positive electrode and the negative one of the Li-ion battery, the transport of the Li+ ions is insured by the electrolyte.

1 Selection of lipase for transesterification in IL We initially selected methyl caffeate and 3-cyclohexyl-1-propanol as substrates for comparative study of the enzyme’s performance in [bmim][NTf2]. Four commercially available lipases, C. antarctica lipase B (Novozyme 435, Novozymes, 30 U mg-1), Rhizomucor miehei lipase (RMIM, Novozymes, 1370 U mg-1), B. cepacia lipases (PS-CI, Wako Pure Chemical, Osaka, Japan, 2560 U mg-1), and Thermomyces lanuginosus lipase (TLIM, Novozymes, 1850 U mg-1) were tested (Table 2).

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