Download RNA Nanotechnology and Therapeutics: Methods and Protocols by Peixuan Guo, Farzin Haque PDF

By Peixuan Guo, Farzin Haque

This quantity encompasses a compilation of innovations and laboratory protocols at once concerning RNA nano know-how and its purposes in nano biotechnology and nano drugs. The chapters during this booklet conceal quite a lot of study equipment that may be simply comprehended and performed in a step by step demeanour by means of graduate scholars and postdoctoral fellows from diversified medical disciplines. the various lined issues comprise a number of biochemical, biophysical and bioinformatics ways for studying RNA constructions and homes; easy methods to study the multi-step meeting means of RNA nano architectures; purification of multifunctional RNA nanoparticles via ultracentrifugation and HPLC; real-time detection of RNA nano constructs in vivo; conjugation of imaging, focusing on and healing modules to RNA scaffold; and layout and characterization of RNA-protein nanostructures. Written within the hugely winning Methods of Molecular Biology sequence structure, chapters comprise introductions to their respective issues, lists of the required fabrics and reagents, step by step, quite simply reproducible laboratory protocols and key pointers on problems troubleshooting and heading off identified pitfalls.

Cutting-edge and sensible, RNA Nanotechnology and Therapeutics: equipment and Protocols propels the sector towards new heights by means of attractive researchers operating within the different fields of actual, engineering, and existence sciences.

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RNA Nanotechnology and Therapeutics: Methods and Protocols

This quantity features a compilation of thoughts and laboratory protocols without delay concerning RNA nano know-how and its purposes in nano biotechnology and nano drugs. The chapters during this publication disguise a variety of study equipment that may be simply comprehended and performed in a step by step demeanour by way of graduate scholars and postdoctoral fellows from various medical disciplines.

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Nasalean L, Baudrey S, Leontis NB, Jaeger L (2006) Controlling RNA self-assembly to form filaments. Nucleic Acids Res 34:1381–1392 13. Afonin KA, Grabow WW, Walker FM, Bindewald E, Dobrovolskaia MA, Shapiro BA, Jaeger L (2011) Design and self-assembly of siRNA-functionalized RNA nanoparticles for use in automated nanomedicine. Nat Protoc 6:2022–2034 14. Patil VS, Zhou R, Rana TM (2013) Gene regulation by non-coding RNAs. Crit Rev Biochem Mol Biol 49(1):16–32 15. Storz G, Vogel J, Wassarman KM (2011) Regulation by small RNAs in bacteria: expanding frontiers.

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35. 36. 37. 38. 39. 41 open complex formation by Escherichia coli RNA polymerase at the T7A1 promoter. Proc Natl Acad Sci U S A 102:4706–4711 Majdalani N, Cunning C, Sledjeski D, Elliott T, Gottesman S (1998) DsrA RNA regulates translation of RpoS message by an antiantisense mechanism, independent of its action as an antisilencer of transcription. Proc Natl Acad Sci U S A 95:12462–12467 Lease RA, Cusick ME, Belfort M (1998) Riboregulation in Escherichia coli: DsrA RNA acts by RNA:RNA interactions at multiple loci.

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